Microtubule-mediated cell protrusion networks direct three-dimensional tissue morphogenesis via intercellular transport of signalling molecules and organelles

The regulation of tissue morphogenesis is a fundamental aspect of animal development. Our recent findings indicate that the dynamics of microtubule-mediated cell protrusions, known as the Interplanar Amida Network (IPAN), are crucial for three-dimensional (3D) tissue morphogenesis in Drosophila pupal wings. This is achieved through coordinated mitoses between two-layered wing epithelia. To decipher the molecular and cellular mechanisms driving IPAN-mediated 3D tissue growth, we aim to investigate how BMP signaling leads to coordinated growth and how mitochondrial transport can maintain tissue homeostasis by employing in vivo live imaging and genetic manipulation tools. Based on these observations, we will unveil novel mechanisms of cell-cell communication accompanying cell shape changes. These approaches will enhance our understanding of the mechanisms driving 3D tissue morphogenesis and have implications for both physiological and pathological processes during animal development.